Efficacy of essential oils as antiseptics on the productive characteristics of the mulberry silkworm Bombyx mori L.
Keywords:Antiseptic, Bombyx mori, Nutritional enhancers, Essential oils
The study aimed to test the efficacy of three essential oils (basil Ocimum basilicum L., lemon Citrus limon (L.) Osbeck, and thyme Thymus vulgaris L.) as disinfectants, including their positive and negative effects, on the biological and productive parameters of the silkworm Bombyx mori. Biological parameters: basil oil treatment at 2000 ppm the highest significant 5th instar larval weight and pupal weight were 2.226 g and 0.787 g. In addition, at the same concentration, recorded the lowest significant mortality percentage and 5th instar larval duration, were 0.787 g and 5.09% respectively. On the other hand, lemon and thyme oils at 4000 ppm come in the second place the same parameters, compared to the control and the chemical disinfectant. While it is equal to the concentration of 8000 ppm for the oils tested in all biological parameters with the control and chemical disinfectant. Economical parameters: basil oil at 2000 ppm and lemon and thyme oils at 4000 ppm had the highest significance for cocoon weights, cocoon shell weight, and silk productivity, which were 1.203 g, 0.220 g, 2.34 cg for basil oil, 1.139 g, 0.210 g, 2.367 cg for lemon oil and 1.265 g, 0.216 g, 2.397 cg for thyme oil, compared with control and disinfectant chemical groups (0.993 g, 0.157 g, 1.49 cg and 0.991 g, 0.160 g, 1.68 cg, respectively). The highest significant difference of cocoon percentages was seen with basil oil at 2000 ppm, compared to the other treatments.
Yilmaz O, Erturk YE, Coskun F, Ertugrul M. Biology of silkworm (Bombyx mori) in Turkey. Conference Pap, 2015.
Chopade P, Raghavendra CG, Mohana Kumar S, Bhaskar RN. Assessment of diseases in Bombyx mori silkworm – A survey. Global Trans Proceed. 2021; 2: 133-136.
Singh M, Singh DM. Effect on productivity of silk due to choice of different feeding plants (Tarminalia Arjuna –Tasar silkworm, mulberry plant –Bombyx mori) in Ambikapur District Surguja Chhattisgarh. Int J Dev Res. 2020; 10(4): 34952-34959.
Greiss H, Petkov N, Yungen M. Optimized rearing conditions for silkworm Bombyx mori L. egg production under Egyptian conditions. Philippine J Sci. 2002; 131(2): 137-141.
Isman MB. Bioinsecticides based on plant essential oils: A short overview. Z Naturforsch. 2020; 75(7-8)c: 179-182.
Mukarram M, Khan MMA, Zehra A, Choudhary S, Aftab T, Naeem M. Biosynthesis of lemongrass essential oil and the underlying mechanism for its insecticidal activity. Med Arom Plants. 2021; 429-443.
Tripathi AK, Upadhyay S, Bhuiyan M, Bhattacharya PR. A review on prospects of essential oils as bio pesticide in insect-pest management. J Pharmacogn Phytother. 2009; 1(5): 52-63.
Hamid AA, Aiyelaagbe O, Usman LA. Essential oils: Its medicinal and pharmacological uses. Int J Curr Res. 2011; 3(2): 86-98.
Omar AS, Fathy M. Dina. Evaluation of controlling silkworm bacterial diseases using propolis extract and cinnamon oil. J Agric Chem Biotechnol. 2016; 7(7): 213-218.
Sampath A, Sujatha K, Suryam A, Singaracharya MA. Antibacterial activity of phyto essential oils on Flaccherie causing bacteria in the mulberry silk worm, B. mori. L. J Pharm Biolog Sci. 2015; 10(5): 29-33.
Regnault-Roger C, Vincent C, Arnason JT. Essential oils in insect control: Low-risk products in a high-stakes world. Annu Rev Entomol. 2012; 57: 405-424.
Choudhury SN, Vajczikova I. Variations in the essential oil composition of Persea bombycina (King ex Hook. f.) Kost and its effect on muga silkworm (Antheraea assama Ww) - A new report. Indian J Chem. 2003; 42B: 640-647.
Singh P, Prasad S, Upadhyay VB. Effect of the essential oil Aloe vera on the cocoon traits of multivoltine mulberry silkworm Bombyx mori Linn. Eur J Biol Sci. 2014; 6(1): 7-12.
Krishnaswami S. New technology of silkworm rearing II. Central Sericulture Research and Training Institute, 1978.
Goudar KS, Kaliwal BB. Effect of cortisone and hydrocortisone on the biochemical changes in the fat body and haemolymph of the silkworm, Bombix mori L. Int J Industr Entomol. 2001; 2(2):181-184.
Krishnaswami S, Narasimhanna MN, Suryanarayan SK, Kumararaj S. Manual on sericulture: Silkworm Rearing. FAO Agricultural Services Bulletin. 1973; 15(2): 42-43.
Chattopadhyay C. Wilt Complex of Castor (Ricinus communis L.): Role of reniform (Rotylenchulus reniformis Linford and Oliveira) nematode. J Oilseeds Res. 1995; 12(2): 203-207.
Snedecor GW, Cochran WGS. Statistical methods applied to experiments in agriculture and biology. 6th edn. Ames, Iowa: Iowa State University Press, 1967.
Marwa NM, Eman MH. Efficiency of some therapeutic essential oils as antibacterial and antioxidants on some biochemical parameters of infected silkworm Bombyx mori L. Egypt Acad J Biol Sci. 2017; 10: 91-98.
Pachiappan P, Aruchamy MC, Ramanna SK. Evaluation of antibacterial efficacy of certain botanicals against bacterial pathogen Bacillus sp. of silkworm, Bombyx mori L. Int J Industr Entomol. 2009; 18: 49-52.
Sakkas H, Papadopoulou C. Antimicrobial activity of basil, oregano, and thyme essential oils. J Microbiol Biotechnol. 2017; 27(3): 429-438.
Kumar A, Dubey NK, Srivastava S. Antifungal evaluation of Ocimum sanctum essential oil against fungal deterioration of raw materials of Rauvolfia serpentina during storage. Indust Crops Prod. 2013; 45: 30-35.
Falowo AB, Mukumbo FE, Idamokoro EM, Afolayan AJ, Muchenje V. Phytochemical constituents and antioxidant activity of sweet basil (Ocimum basilicum L.) essential oil on ground beef from Boran and Nguni cattle. Int J Food Sci. 2019: 2019: 1-8.
Carrasco H, Raimondi M, Svetaz L, Liberto MD, Rodriguez MV, Espinoza L, et al. Antifungal activity of eugenol analogues. Influence of different substituents and studies on mechanism of action. Molecules. 2012; 17: 1002-1024.
Soković M, Glamočlija J, Marin PD, Brkić D, van Griensven LJLD. Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. Molecules. 2010; 15: 7532-7546.
Helaly W, Elyamani E, Saad M. Influence of some plant extracts as antiseptics to control bacterial and fungal diseases of silkworms, Bombyx mori L. Zagazig J Agric Res. 2020; 47(3): 307-317.
Karthikairaj K, Isaiarasu L, Sakthivel N. Efficacy of some herbal extracts on microbes causing flacherie disease in mulberry silkworm, Bombyx mori L. J Biopest. 2014; 7: 89-93.
Saad MSI, Elyamani EMY, Helaly WMM. Controlling of bacterial and fungal diseases that contaminating mulberry silkworm, Bombyx mori by using some plant extracts. Bull Nat Res Centre. 2019; 43: 172.
Mona M Mahmoud. Effect of certain evaluated plant crude extracts on the productivity of silkworm (Bombyx mori L.). J Adv Agric Res. 2014; 19(1): 50-59.
Dewer Y, Mona MM. Effectiveness and safety of some essential oils of aromatic plants on the growth and silk production of the silkworm Bombyx mori L. J Entomol Zool Stud. 2014; 2(2): 81-86.
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